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Use of Monoclonal-Antibodies to Human Hla-Ii Antigens for the Detection of Bovine B-Lymphocytes and Macrophages

DUSINSKY R, SIMON M, UJHAZYOVA J, POLAKOVA K
Veterinarni Medicina 37, 1992, 549-554

The crossreactivity of mouse monoclonal antibodies [MoAbs] [Tab. I] prepared against human HLA-DR and HLA-DP antigens was studied in various bovine cells: lymphocytes from lymph nodes and peripheral blood, adherent [B] and nonadherent [T] lymphocytes, monocytes, granulocytes and platelets. In the immunofluorescence test, MoAbs Bra13, Bra14, Bra20, Bra22, Bra30, Bra70, HL-38 reacted with bovine B lymphocytes and monocytes, but not with other tested cells [Tab. III, IV]. These antibodies, except Bra22, were positive with B lymphocytes in the complement dependent cytotoxic test [Tab. II]. The similarity of the bovine antigens and HLA-DR antigens determined by used MoAbs was also proved by immunoblotting. Monoclonal antibodies Bra38 and BraFB6 did not react with the bovine cells and separated antigens. The epitope [HLA-DR] recognized by the antibody Bra38 is probably absent in cattle. The presence of HLA-DP analogue determined by the antibody BraFB6 has not been confirmed. The crossreactive MoAbs could be used for the detection of B lymphocytes and macrophages in veterinary immunology


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