The Stimulation of Serum Antibody Development in Pigs Induced by Means of Oral, Subcutaneous and Combined Administration of Live Porcine Rotavirus Or Im Inoculation of Inactivated Porcine Rotavirus in Oil Adjuvant

Veterinarni Medicina 36, 1991, 477-483

Only live vaccines prepared from attenuated strains have been used for the specific prophylaxis of rotavirus infections in pigs. These vaccines are administered to sows per os or parenterally to increase the content of antibodies in the blood serum, colostrum and milk, and in this way to provide for the better passive protection of suckling piglets through the maternal antibodies, or to induce the active immunity by pig vaccination. The data on the efficiency of live vaccines administered in both ways differ as to their ability to stimulate significantly increases in the actual levels of antibodies in sows and also as to the possibility of inducing the protection of vaccinated pigs from virulent virus infection. The objective of our trials was to compare the intensity of antibody response evoked by pig vaccination with live virus if the virus was implanted in different ways, and by vaccination with inactivated virus emulsified in oil adjuvant. The live vaccine consisted of a suspension of porcine rotavirus, strain OSU/6, cultivated in MA-104 culture medium with the content of 10(7) TKID50. ml-1, the inactivated vaccine was the identical virus suspension inactivated by an addition of 0.2% formaldehyde during 24 hours at a temperature of 37-degrees-C, emulsified in oil adjuvant by means of an ULTRATURAX equipment at a 4:1 ratio. Thirty wealings from a conventional herd, at the age of 10 to 12 weeks, divided into five groups with six animals in each, were vaccinated as follows: each weanling of group 1 was administered 2 ml of live virus s. c.

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