Early immunological events in germ-free piglets monoassociated with nonpathogenic or virulent strain of Salmonella typhimurium

Veterinarni Medicina 45, 2000, 125-128

An early response (6 and 24 hrs) of germ-free miniature piglets to oral treatment with virulent LT2 strain or avirulent SF1591 rough mutant of S. typhimurium was studied. Six hours postinfection, bacteria colonized the gut except for the rectum and penetrated into the mesenteric lymph nodes. In addition, LT2 strain was found in the spleen, blood and lungs. The virulent strain damaged ileal enterocytes and caused immigration of myeloid cells into the villus lamina propria. Virulent but not nonpathogenic bacteria in lymphatic organs (but not in the gut) were stained with mAb recognizing heat shock protein HSP65. No significant change in the distribution of lymphocyte subpopulations could be found six hours postinfection

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