The Effects of Zindep Inj Preparation on Zincaemia, T-Lymphocytes and Beta-Lysine in Pregnant Dairy-Cows

Veterinarni Medicina 36, 1991, 641-648

The effects of zinc administration at a rate of 3 mg/kg lw, in the preparation Zindep inj. ad usum vet. (Biotika, Slovenska L'upca) were evaluated as exerted on zinc concentrations in the blood serum of 16 dairy cows in the middle of the 7th month of pregnancy. With respect to zinc injection, T-rosetted lymphocytes and beta-lysine activity were in estigated. Blood was collected from all dairy cows from v. jugularis before the preparation was administered, on days 2, 5, 8, 15, 30 and 60 after Zindep administration. Atom absorption spectrophotometry, applying a flame technique on a Perkin Elmer 1100 apparatus (Bires, 1986), was used to determine Zn concentrations in the blood serum of all dairy cows. T-lymphocytes were determined by a rosette test after Paul et al. (1977), and beta-lysine was detected spectrophotometrically after Bucharin et al. (1987). Zinc dynamics in the blood serum of dairy cows is presented in Fig. 1. The starting values of zinc in the test cows were 9.68 +/- +/- 2.30-mu-mol/l and in the control ones 10.15 +/- 1.27-mu-mol/l. Zincaemia of experimental dairy cows was significantly lower (P < 0.01) on day 2 after Zindep administrations, in comparison with the control group. A significant increase in zinc concentrations in the blood serum of experimental animals. in comparison with the control ones, was observed from day 8 to day 60 (P < 0.01). The maximum zincaemia values were recorded in experimental dairy cows within days 15 and 30 (15.65 +/- 3.33, and/or 14.55 +/- 2.10-mu-mol/l). The variance of the average values of zinc concentrations in the control animals ranged within the lower limit of reference values during the observation period (9.50 - 11.13-mu-mol/l). The T-Ly activity is presented in Fig. 2. The starting value of T-Ly made 38.45 +/- 2.49 % in experimental dairy cows and 39.32 +/- 2.08 % in control ones. A statistically significant increase in T-Ly in experimental animals, if compared with the control, was observed on day 2 after Zindep administration (P < 0.01). The percentage of T-rosetted Ly also increased on day 5 in the experimental group. The maximum values of T-Ly were recorded on days 8 and 15 in experimental animals (49.96 +/- +/- 2.38 and 47.74 +/- 2.02 %, resp.). A significantly higher percentage of T-Ly was recorded within days 2 and 60 in experimental cows if compared with the control ones (P < 0.01, and/or P < 0.05). The dynamics of T-rosetted Ly was steady in the control dairy cows during the observation period (the variance of the values made 38.34 +/- 1.76 to 40.12 +/- 2.41 %). The values of beta-lysine in dairy cows are presented in Fig. 3. The activity of beta-lysine in the blood serum before Zindep administration was 57.14 +/- +/- 10.98 % in experimental dairy cows and 67.7 +/- 9.31 % in the control ones. In comparison with the starting values, an increase in the beta-lysine activity was observed as soon as on day 2 in the two studied groups. In the following period the activity of beta-lysine also had the same pattern in the experimental and control dairy cows. The highest value of beta-lysine was recorded on day 60 in the experimental group (87.84 +/- 2.70 %) and on day 5 of experiment in the control (76.52 +/- 11.31 %). Statistically significant differences in the beta-lysine activity between the experimental and control animals were confirmed on day 60 (P < 0.05). The above-mentioned results demonstrate positive effects of zinc administration in form of the Zindep inj. preparation on zincaemia levels in pregnant dairy cows. The zinc injections increased cell-mediated responses in the experimental animals that were evaluated by means of T-rosetted Ly. The effects of Zindep administration on the beta-lysine activity were not so clear in the blood serum of the experimental animals

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