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A decrease of sister chromatid exchanges induced by carbon tetrachloride in cultured sheep peripheral lymphocytes by vitamin E and selenium

SIVIKOVA K, DIANOVSKY J
Veterinarni Medicina 45, 2000, 322-326

The protective effect of vitamin E and selenium was studied for their possibility to decrease the sister chromatid exchanges (SCE) induced by carbon tetrachloride (CCl4) in cultured sheep peripheral lymphocytes in vitro. Whole blood cultures of two healthy male lambs were treated with carbon tetrachloride at the concentrations of 2, 4, 8 and 16 mug/ml for the last 24 h of cultivation and subsequently with the same dose of CCl4 and both nutrients vitamin E and selenium. After treatment of sheep peripheral lymphocytes with CCl4 for 24 h, no dose-dependent increase of SCE was obtained. The statistically significant elevations in the mean of SCEs were seen at the concentrations of 4 and 8 mug/ml (p < 0.001 and p < 0.01, respectively) in both donors. No significant effects on SCE increases were recorded after the treatment with CCl4 at the lowest and the highest dose. The later was also shown to reduce the proliferation index (PI) in both donors (p < 0.001 and 0.01). Vitamin E and selenium significantly inhibited CCl4-induced SCEs at the concentration of 4 mg/ml in comparison to the corresponding non-protective dose (p < 0.001). No or a weak protective effect was achieved in the cultures with CCl4 at the concentrations of 2 and 8 mg/ml supplemented with both nutrients in comparison to the cultures exposed to CCl4 alone. No improvement of cell cycle kinetics was achieved


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