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Effects of heat-processing of milk on validation characteristics of the Charm AIM-96 and Delvotest SP screening tests

SCHLEGELOVA J, RYSANEK D
Veterinarni Medicina 45, 2000, 201-207

Two commercial screening tests (Charm AIM-96 and Delvotest SP) for the detection of antimicrobial residues in milk were evaluated using samples of heat-processed milk. Heating to 85 degrees C for 5 min had no effect on detection limits for selected antimicrobial agents diluted in milk including penicillin, streptomycin, neomycin, chlortetracycline, cloxacillin, chloramphenicol and sulphadiazine. Heat degradation of the interfering natural inhibitors (lysozyme, IgG) resulted in considerable changes of almost all characteristics of validation of raw and heat-processed milk in both the tests. The value of true specificity increased from 96.55% for Charm AIM-96 and 90.62% for Delvotest SP to 100.0%. The most marked change occurred in the cutoff specificity which is decisive for correct scoring of milk with the concentrations of antimicrobial residues
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