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PAPER SELECTED

Immunochromatographic detection of bovine rotavirus using egg yolk antibodies

RESCHOVA S, FRANZ J, STEPANEK J, ROZKOSNA A
Veterinarni Medicina 45, 2000, 33-37

An immunochromatographic test (ICT) for the detection of bovine rotavirus in fecal samples using the specific anti-rotavirus colloid gold-bound immunoglobulin isolated from egg yolks (IgY) of immunized hens has been developed. The yield of IgY was 100 to 150 mg per yolk and ELISA antibody titers ranged between 50 000 and 100 000. ICT was used for 130 fecal samples collected from calves aged from 1 to 50 days. A comparison with ELISA showed 90.7% agreement. The values of specificity and sensitivity were 97.3% and 82.1%, respectively. In an analogous comparison with Dot-ELISA, the agreement, specificity and sensitivity values obtained for ICT were 96.9%, 96.4%, and 97.8%, respectively. Detection of rotavirus double-stranded RNA by agar-gel electrophoresis resulted in 90.3% agreement with ICT


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