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Production of anti-sperm antibodies associated with genital Chlamydia infection in rabbits

POSPISIL L, DIBLIKOVA I, VEZNIK Z, ZRALY Z, HOROVA I, BUDIKOVA M
Veterinarni Medicina 45, 2000, 163-170

Male Californian rabbits (n = 10) with well developed testicles were inoculated intratesticularly with 0.5 mi suspension of Chlamydia psittaci (ewe abortion strain PK-5082) divided in both testicles. Control rabbits (n = 5) received the same volume of physiological saline. Blood samples for serological examinations were collected from the ear vein one week before and weekly intervals after the inoculation. Antibodies to chlamydiae and anti-sperm antibodies were determined by ELISA. The results of ELISA for each of the rabbits were plotted against time and mean data for the group were processed by the kernel smoothing (Hardle, 1990). It is evident from the results that the intratesticular inoculation of chlamydiae induced the formation of specific anti-chlamydial antibodies. At the same time formation of antisperm antibodies was observed in most of the inoculated rabbits. The dynamic of titres of two types were similar


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