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PAPER SELECTED

The use of egg yolk immunoglobulin in the diagnostics of canine parvovirus infections

POKOROVA D, FRANZ J, STEPANEK J
Veterinarni Medicina 45, 2000, 49-54

ELISA procedures for the detection of canine parvovirus-2 (CPV-2) in faecal samples and of CPV antibodies in blood serum or whole blood are described. The basic component of the two procedures is solid phase-bound egg yolk antibodies to CPV-2 - IgY. Comparative trials of ELISA and haemagglutination test using 96 faecal samples demonstrated a 75% agreement of results. A comparison of ELISA and haemagglutination inhibition test using 149 canine blood serum samples yielded agreeing results in 87.9% of the animals. The usability of ELISA for the demonstration of antibodies to CPV-2 in whole blood was tested by examination of 70 and 36 samples collected from adult dogs and vaccinated pups, respectively. The differences between the blood serum and whole blood titres were insignificant (p > 0.05) and their correlation was expressed by the coefficient r = 0.870


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