vetmed

PAPER SELECTED

Biochemical markers of dioxin-like toxicity and oxidative stress in hepatic microsomes of dream (Abramis brama) and perch (Perca fluviatilis) in the Elbe River

MACHALA M, HILSCHEROVA K, RUBINOVA R, ULRICH R, VYKUSOVA B, KOLAROVA J, MACHOVA J, SVOBODOVA Z
Veterinarni Medicina 45, 2000, 195-199

Biochemical markers of dioxin-like toxicity and oxidative stress were investigated in hepatic microsomal fractions of bream (Abramis brama) and perch (Perca fluviatilis) collected at six sites of the Elbe river (Czech Republic). Concentrations of polychlorinated biphenyls (PCBs) and organochlorine pesticides were determined in pooled muscle samples to screen contamination rates of the sampling sites. Induction of the cytochrome P4501A-dependent 7-ethoxy-resorufin O-deethylase (EROD) activity was used as the biomarker of dioxin-like toxicity, and the activation of microsomal glutathione 5-transferase (mGST) and in vitro susceptibility to lipid peroxidation were chosen as the biochemical markers of oxidative stress in fish. The concentrations of PCBs and DDT metabolites were higher in bream than perch tissues


FULL PAPER on request
« back