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PAPER SELECTED

Differentiation of verotoxigenic strains of Escherichia coli isolated from piglets and calves in the Czech Republic

ALEXA P, HAMRIK J, SALAJKA E, STOURACOVA K, RYCHLIK I
Veterinarni Medicina 45, 2000, 39-43

A set of 451 and 115 strains of Escherichia coli isolated from piglets and calves affected by diarrhoea, respectively, were tested for verotoxin production and type. The production was tested on Vero cells and the type was identified by PCR. The production of verotoxin was demonstrated in 189 of the porcine strains and 13 of the bovine strains. Most of the porcine verotoxigenic strains belonged to the serogroups O139, O141, O157, and O138. Positive for verotoxin production were 83.3% and 78.2% of the strains of the serogroups O139 and O141, respectively. The type VT2e was identified in 184 strains and a sequence corresponding to VT2c was found in 2 strains. However, the latter yielded negative results in the Vero cells test. Furthermore an unidentified toxin type was demonstrated in three strains positive in the Vero cell culture. The bovine verotoxigenic strains belonged to the serogroups O20, O26, O103, O111, and O157. Both VT1 and VT2 were produced by 6 (46.2%) of the 13 verotoxigenic strains. Only one verotoxin type was produced by each of the remaining verotoxigenic strains


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