The interaction of two forms of glutamic acid with ammonia in sheep

Veterinarni Medicina 44, 1999, 229-232

In our experiments, 20 Slovak Merino sheep 36.5 +/- 4.0 kg b.w., aged 3.5 years were used. They were starved 12 h before the experiment and for 6 h after the initial administrations of the agents. Sheep had free access to water throughout the period. Control sheep received in addition to urea (0.5 g/kg b.w.), an equimolar quantity of 15% nonbuffered glutamic acid solution and the experimental sheep received the same quantity of sodium hydroxide buffered glutamic acid solution. The laboratory indices demonstrated that nonbuffered glutamic acid in contrast to sodium hydroxide buffered glutamic acid was able to protect sheep from ammonia intoxication induced via p.o. administration of urea (see Tab. I). The experiments demontrated a significant trend towards higher concentrations of ammonia (P < 0.01) and lower values of pH (P < 0.01) in the rumen fluid and lower concentrations of ammonia in the blood (P < 0.01) of sheep with nonbuffered glutamic acid in contrast to sodium hydroxide buffered glutamic acid, especially 60 min and more after the administration of agents. We did not however register any significant differences in the dynamics of urea concentrations between both groups of sheep in blood plasma throughout the period of experiments (P < 0.05). Clinical observations of animals demonstrated that sodium hydroxide buffered glutamic acid was not able to protect sheep satisfactorily against ammonia intoxication. Sheep in this group showed some intoxication symptoms and one died, in contrast to sheep in the control group with nonbuffered glutamic acid

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