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PAPER SELECTED

Identification of toxigenic strains of Pasteurella multocida by PCR and biological methods

SATRAN P, BARTOS M, NEDBALCOVA K, RYCHLIK I, KONSTANTINOVA L, ULMANN L
Veterinarni Medicina 44, 1999, 275-278

Sensitivity and specificity of methods for the identification of toxigenic strains of Pasteurella multocida were compared. The production of dermonecrotoxin was determined by a skin test in guinea pigs, a cell culture test using bovine embryonic lung cells, and PCR. The highest sensitivity and specificity were found for PCR that allows the identification of strains producing only small amounts of the toxin in vitro that are not detectable by biological methods. However, strains producing structurally different toxins with the same biological effect are not identifiable by this technique owing to its high specificity. Therefore, biological and particularly cell culture tests are necessary for a comprehensive assessment of toxigenicity of individual strains


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