vetmed

PAPER SELECTED

The use of immunomagnetic separation in detection of Salmonella and Listeria from foodstuffs

KARPISKOVA R, HOLASOVA M
Veterinarni Medicina 44, 1999, 225-228

Immunomagnetic separation (IMS) and conventional methods (ISO guidelines) were used for the routine testing of various foodstuffs for the presence of Salmonella spp. and Listeria spp. The total of 408 food samples (milk, egg, and meat products, raw meat, confectionary, vegetable and fruit products) were examined for the presence of Salmonella spp. and 33 samples (8%) were positive using conventional methods. Using IMS 49 (12%) of them were positive. A higher number of false negative results was obtained in milk powder using conventional methods compared to the IMS technique. The sensitivity of IMS was higher than in conventional methods (ISO 6579). The statistical differences were of great significance especially when using Rappaport Vassiliadis medium (p = 0.01). The number of 375 food samples (milk products, raw meat and meat products) were examined for the presence of Listeria spp., 81 samples (21.6%) were positive using conventional methods and 91 (24.3%) were positive using IMS. The statistical differences were significant when using the USDA method (p = 0.05) and there were no significant differences between IMS and FDA method. Our results demonstrated that the IMS technique was either more sensitive than or as sensitive as the conventional detection procedures and saved at least one day eliminating the selective enrichment step


FULL PAPER on request
« back