Di-2-ethylhexyl phthalate and di-n-butyl phthalate in the tissues of pigs and broiler chicks after their oral administration

Veterinarni Medicina 44, 1999, 61-70

The distribution and cumulation of the most toxic phthalates di-2-ethylhexyl phthalate (DEHP) and di-n-butyl phthalate (DBP), were followed up in the tissues of pigs and broiler chicks after their oral application. Besides. the presence of mono-2-ethylhexyl phthalate (MEHP), a metabolite of DEHP, in the blood and urine was investigated. Phthalates were orally administered to pigs, at a dosage of 5 g each, daily, for 14 days (dissolved in edible oil and added to the first part of the feed), and to broilers at 100 mg each, daily (in a gelatine capsule) directly to the crop. III pigs, phthalates were analyzed in the liver, kidneys, lungs, brain, heart, muscle, renal and subcutaneous fats. While in broilers, they were examined in the muscle, skin, liver and mesenterial fat tissue. The analysis was performed just after the 14 days of application and then 14 and 28 days after the last day of phthalate application. During the experiment, the animals showed no clinical symptoms of disease. DEHP was present in the blood and urine of pigs, whereas broiler's blood was positive for MEHP. The lipophilic character DEHP and DBP was confirmed. After 14 days of DEHP and DBP application. the highest concentration was found to be in the muscle and adipose tissue of the experimental pigs (DEHP: 1.22 and 1.43 in the muscle, 14.37 and 12.20 in the adipose tissue: DBP: 1.44 and 1.83 in the muscle, 9.42 and 9.64 in the adipose tissue - in mg/kg of the original sample). Total amount of DBP in broilers was eight fold less than DEHP. DBP was equally distributed in all the followed tissues, while DEHP was mainly accumulated in the fat tissue. After 14 days of application, DBP content in the skin was 0.9, muscle 0.19, mesenterial fat 3.13 and in the liver 0.27 mg/kg. DEHP content in the skin was 8.28, muscle 1.93, mesenterial fat tissue 18.20 and in the liver 0.32 mg/kg of the original sample (average from six experimental bl oilers). Fourteen and twenty eight days after the last application, a high persistence of both phthlates was detected in the body of broilers. The adipose tissue is the best indicator for the presence of phthalates in the organism. The tissues of control pigs and broilers contained both DEHP and DBP in detactable concentrations and also the presence of phthalates originating from the contaminated leed was confirmed. All samples collected from the feed mixtures currently available on the market, which were used for the feeding ol experimental and control animals, contained DEHP and DBP (DEHP 0.24-1.77, DBP 0.06-2.37 mg/kg of feed). These are original results since phthalate fate in the body of livestock animals had not been studied previously. Confirmation of the presence and distribution of toxic phthalates in the body of livestock animals after peroral administration is very important for the safety of raw ingredients slid animal food products

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