vetmed

PAPER SELECTED

Leukocyte counts and lymphocyte subpopulations in the peripheral blood of pygmy goats from herd infected with Mycobacterium avium subspecies paratuberculosis

FALDYNA M, TOMAN M, PAVLIK I
Veterinarni Medicina 44, 1999, 259-262

The aim of this paper was to study the total and differential leukocyte counts and lymphocyte subpopulations in the peripheral blood of pygmy goats. Lymphocyte subpopulations were enumerated by flow cytometry using monoclonal antibodies against surface antigens of bovine or ovine leukocytes, but not all of these were found to cross-react. Therefore, only CD2, 5, 8, 11a/18, 18, 14 and B-cells were calculated. Goats were divided into two groups according to age. Three of them were 4-6 months old and eight were adult (3-6 years). Difference in some parameters between age-related groups were found. The group of adult goats had highly statistically significant (p < 0.01) more neutrophils and less lymphocytes than the young ones. Adult goats had also statistically highly significant (p < 0.01) more T-lymphocytes and less B-lymphocytes. In addition, each goat in both groups was identified to be infected with Mycobacterium avium subsp, paratuberculosis, but without clinical signs of the disease. Results of both goats did not differ from the other animals in the group


FULL PAPER on request
« back