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PAPER SELECTED

Detection of human protein C gene integration in transgenic rabbits by polymerase chain reaction

CHRENEK P, VASICEK D, MAKAREVICH A, GAJARSKA T, GASTNEROVA I, BULLA J
Veterinarni Medicina 44, 1999, 79-82

From a total of 200 rabbit pronuclear stage zygotes microinjected with WAP-hPC gene (mouse whey acid protein promoter - human protein C) were obtained 21 liveborn descendants after transfer to recipients. The integration of the WAP-hPC gene in the genome of born rabbits was determined in samples of DNA, isolated from tissue ol blood by PCR method. The integration was confirmed in 2% (4/200), or in 19% (4/21) of F-0 rabbits, in 11% (5/46) of F-1 and in 31% (4/13) of F-2 generation using two primer sets


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