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PAPER SELECTED

Effect of busulphan on growth and development of the chicken embryos

SMYKALOVA S, KOTRBOVA A, TREFIL P
Veterinarni Medicina 43, 1998, 105-109

We have studied the effects of busulphan dissolved in sesame oil in the dose of 100 mu g/50 mu l per egg on the growth and development of chicken embryos. Busulphan was injected into egg yolk. We found that the higher dose of busulphan had a negative influence on chicken embryo development. The size of treated embryos was significantly reduced (about 1/3 in comparison with the control embryos) after 8 days of incubation. The overall number of surviving embryos was significantly higher in the control group. The gonads of busulphan treated embryos were significantly smaller in comparison with untreated embryos. The total number of gonad cells (somatic and primordial germ cells) was reduced in the busulphan treated embryos more than 50%. Hatched busulphan treated cocks showed retarded secondary sexual characteristic in 12 weeks of age


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