Isolation of Lactococcus garvieae species from bovine mastitis

Veterinarni Medicina 43, 1998, 371-374

Bovine mastitis is caused mostly by streptococci or staphylococci. From the streptococcus-related group the Lactococcus garvieae is mentioned in connection with the illness. This species is able to grow both at 10 and 45 degrees C (L. garvieae is the only lactococcus which grows at 45 degrees C) and in the presence of 6.5% NaCl, which are the key characteristics of enterococci and should be in this way misidentified as a member of the genus Enterococcus. On the other hand, L. garvieae did not grow at pH 9.6 and did not react with streptococcal grouping antigen for group D. The paper presents the characteristics originated from the five cultures isolated at the end of 1997 year in North-east Moravia. Recent results of tests based on Czech commercial identification kits (STREPTOtest 16, STAPHYtest 16 and ENCOCCUStest) should help with the identification of the pathogen from animal clinical sources because our results were almost identical for all isolates. Positive pyrrolidonyl arylamidase reaction distinguishes L. garvieae from pediococci, leuconostocs or viridans streptococci and together with positive leucine aminopeptidase from aerococci. Members from genus Globicatella do not grow at 10 and 45 degrees C, members from genus Facklamia have negative hydrolyse of esculin in comparison with L. garvieae. Species differentiation of L. garvieae from the other lactococci is based on positive pyrrolidonyl arylamidase and arginine dihydrolase reactions and acid production from ribose and melibiose. According to our observation L. garvieae is no rare species, but probably with regard to lack of information about the taxon it can be commonly identified as viridans streptococcus or as enterococcus. Two isolates were deposited in the Czech Collection of Microorganisms as CCM 4682 and CCM 4683

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