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A densitometric analysis of alkaline phosphatase and aminopeptidase M in the jejunum enterocytes of the piglets with retarded growth

LENHARDT L, DUDRIKOVA E
Veterinarni Medicina 43, 1998, 75-79

The activity of alkaline phosphatase and aminopeptidase M in the jejunum enterocytes was determined in 3 month old clinically healthy piglets with normal (12 animals, average body weight 30.7 kg) and retarded growth (12 animals, average body weight 16.9 kg). A densitometric analysis was used. A marked decrease in an average density of the alkaline phosphatase - Dx 51.3 as well as in the density/area ratio - DAx 1.48 was observed in the animals with retarded growth. This was in contrast with the results from weaned piglets with normal growth - Dr 94.7 and DAx 2.33, respectively. Similar results were observed during evaluation of aminopeptidase M (Dx 35.2 and DAx 1.05 versus Dr 81.62 and DAx 2.12, respectively). The obtained results point to a relationship between the amount of the investigated enzymes found in the microvillous zone of enterocytes and the functional state of enterocytes. These data can be used in the diagnose of the growth disturbances in weaned piglets


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