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PAPER SELECTED

Cytotoxicity and genotoxicity of cadmium (CdCl2) investigated on a rat liver epithelial cell line (Anr-4 cells) and Chinese hamster lung fibroblasts (V 79 cells)

HURNA E, HURNA S
Veterinarni Medicina 43, 1998, 365-369

The cytotoxic effects of cadmium on Anr-4 cell line (rat liver epithelial cells) were assessed by neutral red uptake assay (NR), determination of protein and activity lactate dehydrogenase (LDH) in the medium. Cadmium chloride decreased the viability of cells in a concentration dependent manner. At 25 mu M/1 Cd, the viability was 62.27 +/- 2.78% and at the highest concentration (100 mu M/l CdCl2) was 0.29 +/- 0.33%. The proliferation of cells was determined by the measurement of total protein. At 25 mu M/l Cd, the proliferation was 82.06 +/- 3.34% and at 100 mu M/l Cd 21.80 +/- 1.48%. Leakage of LDH into the medium was used to assess Cd-induced cell membrane damage. LDH activity in the medium of the control cells was 58.09 +/- 2.60% and at 25 mu M/l Cd 72.88 +/- 1.56%. Genotoxic effect was determined by micronucleus test. The fibroblasts of chinese hamster V 79 cell line was used for this purpose. In the control cells, the number of the micronuclei was 5 against the highest concentration (50 mu M/l CdCl2), where the number of micronuclei was 20 (p less than or equal to 0.001)


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