vetmed

PAPER SELECTED

The effectiveness of inactivated, purified and concentrated experimental rabies vaccine for veterinary use: Immunogenic activity

SULIOVA J, BENISEK Z, SVRCEK S, DUROVE A, ONDREJKA R
Veterinarni Medicina 42, 1997, 51-56

The authors prepared an experimental, inactivated, concentrated and highly purified rabies vaccine from the strain Vnukovo-32/107. The purification and concentration (simultaneous - in one operation) was carried out by affinity chromatography. The content of rabies antigen in the vaccine was 4.256 UE/cm(3) (determined by ELISA method). The authors used their own lipoid adjuvant - type oil in water - to resolve the lyophilized vaccine to original volume. It is based on esters of fatty acids. The effectiveness - immunogenic activity - of the experimental preparation was compared with that of commercial rabies vaccines and the reference vaccine by means of the NIH and NRLR methods. The results obtained (2.826 units/cm(3) by the NIH and 3.085 units/cm(3) by the NRLR method) confirmed that the method of affinity chromatography could be used to prepare a rabies vaccine, exhibiting effectiveness superior to commercial preparations available, and complying with the WHO criteria


FULL PAPER on request
« back