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PAPER SELECTED

Determination of tetracyclines in bones, mechanically separated beef, and bones by HPLC method and microbiological agar diffusion tests

KORIMOVA L, MATE D, TUREK P, SOKOL J
Veterinarni Medicina 42, 1997, 129-131

HPLC method with solid phase extraction, and microbiological agar diffusion tests for determination of oxytetracycline (OTC), tetracycline (TC) and chlortetracycline (CTC) in bones, mechanically separated beef and bones were developed. Both mechanically separated bones End bovine flat bones showed the highest yield of tetracyclines by HPLC method. Among tetracyclines, the highest yield was determined in chlortetracycline. The strains of Bacillus subtilis (PH 6.0) and B. stearothermophilus showed the highest sensitivity to the presence of tetracyclines


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