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Testing of TKT medium for Streptococcus agalactiae screening in bulk milk samples

BENDA P, VYLETELOVA M
Veterinarni Medicina 42, 1997, 71-80

A selective medium for quantitative determination of Streptococcus agalactiae in bulk milk samples was tested. Among the medium bases, Edwards medium (EW) supplemented with 6 to 9% (v/v) of carefully washed sheep erythrocytes was proved to be best (Tab. V). The production of sphingomyelinasis C (BHE, Tab. II) or D (COREX, Tab. I) as a supplement supporting the formation of specific hemolytic zones in the medium, was tested in four strains of Staphylococcus aureus and seven strains of Corynebacterium pseudotuberculosis. Submersion aerated cultivation was used for enzyme production


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