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Testing of culture media, for detection of Staphylococcus aureus in bulk milk samples

BENDA P, VYLETELOVA M
Veterinarni Medicina 42, 1997, 9-17

Three common selective agar culture media were tested with respect to detection of Staphylococcus aureus: Baird-Parker agar (BP), Mannitol-Salt-Phenol agar (MAN) and Vogel-Johnson agar (VJ). All media were cultivated at temperatures of 37 degrees C and 43 degrees C. Three groups of samples were used for investigations: 1. pure cultures of 52 collection strains of S. aureus received from the Czech Microorganism Collection (Tab. I), 2. pure cultures of 75 laboratory strains of S. aureus from samples of cow raw milk and 3. 223 bulk samples of cow raw milk. Medium recovery and frequency of medium failure were evaluated, as well as subjective-evaluation of medium selectivity and count feasibility was done. The best results of recovery and failure frequency were observed in BP/37 degrees C for all three groups


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