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PAPER SELECTED

Interactions of Lactobacillus spp and enteropathogenic Escherichia coli under in vitro and in vivo conditions

BOMBA A, NEMCOVA R, KASTEL R, HERICH R, PATAKY J, CIZEK M
Veterinarni Medicina 41, 1996, 155-158

In the present study, the effect of Lactobacillus spp. against enteropathogenic Escherichia coli 08:K88(+)Ent(+) under in vitro and in vivo conditions has been compared and the effect of inoculation of Lactobacillus spp. upon the colonization of both the jejunum and ileum by enteropathogenic E. coli 08:K88(+)Ent(+) in 9 gnotobiotic pigs has been observed. Under in vitro conditions, the strain Lactobacillus spp. showed the inhibition of 2.1 +/- 0.1 mm against enteropathogenic E. coli 08:K88(+)Ent(+). Two days after the inoculation, the enteropathogenic E. coli 08:K88(+)EnC(+), inoculated to the control group of gnotobiotic pigs (E), colonized the mucosa of both jejunum and ileum of gnotobiotic pigs at counts of 6.41 and 6.08 log 10/cm(2), respectively. In experimental group (L-E), the counts of adhered enteropathogenic E. coli in the identical sections of the small intestine, following the inoculation by Lactobacillus spp., amounted to 6.35 and 6.43 log/cm(2), respectively. Tn both groups, numbers of E. coli in the intestinal content of both jejunum and ileum were nearly the same (group E 9.03 and 9.31 log 10/ml


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