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## Colonization of the Digestive-Tract of Gnotobiotic and Conventional Lambs with the Defined Lactoflora

BOMBA A, KRAVJANSKY I, KASTEL R, CIZEK M, KAPITANCIK B, JUHASOVA Z, HERICH R, ZITNAN R, BUCKO V

Veterinarni Medicina 39, 1994, 701-710

The effect of Lactobacillus casei 249/89 on the colonization of the intestinal tract and selected indicators of the metabolic profile of gnotobiotic and conventional lambs from the viewpoint of its possible utilization in the prevention of diarrhoeal syndrome of bacterial etiology in young animals has been studied. Five gnotobiotic and three conventional lambs were used in these studies. The lambs were slaughtered at 3, 6, 10, 15 and 21 days of age. The population of L. casei colonizing the intestinal epithelium was at the age dynamics on average higher in gnotobiotic lambs compared with lactobacilli in conventional lambs (Fig. 1). The significant difference (I, < 0.001) was noted at the age of 3 days (gnotobiotic lambs = 3.40 log 10/cm(2) and conventional lambs = 1.08 log 10/cm(2)). Also the counts of lactobacilli colonizing individual sections of the intestine (Fig. 2) was on average higher in gnotobiots with significant differences in jejunum and colon (p < 0.05). In both groups, the highest number of lactobacilli was observed on the intestinal epithelium in lower sections of the digestive tract. In gnotobiots, the population of L. casei colonizing the duodenum was highest at the age of 3 days (3.49 log 10/cm(2)), in jejunum (3.74 log 10/cm(2)) and in ileum 4.37 log 10/cm(2)) at the age of 6 days and in the colon (4.7 log 10/cm(2)) at the age of 15 days (Fig. 3). In conventional lambs, the population of lactobacilli colonizing individual sections of the intestinum was increasing with age (Fig. 4). The number of L. casei in the intestinal content of gnotobiotic lambs was on average higher than that in conventional animals but the differences were not significant. In both groups, the highest number of lactobacilli was noted at the age of IO days (gnotobiotic lambs = 5.9 log 10/ml

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