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Isoenzymes of Lactate-Dehydrogenase in Purebred and Crossbred Ewes and Rams

SUTIAKOVA I, DALLIOVA K, SUTIAK V, PIJAKOVA N
Veterinarni Medicina 38, 1993, 609-617

In farm animals the questions of clinical and mainly heritable health receive increased attention. In this work the activity of the polymorphous system of lactate dehydrogenase was observed in some pure- and crossbred ewes and rams under field conditions. The isoenzyme of lactate dehydrogenase were separated on 5.5 % polyacrylamid gel as reported Dietz, Lubrano (1967). Age- and sex-related determinations of the isoenzyme spectrum of lactate dehydrogenase revealed increased LD3 activity in yearling ewes, and increased LD4 and LD5 activity in yearling rams (Tab. I). Significant differences of LD4 isoenzyme activity (P < 0.05) were observed between the Stavropol Merino and the Stavropol Merino x Merino x Corriedale crossbreds (Tabs II and III). In the blood plasma of Stavropol Merino rams the following quantities of LD isoenzymes were determined: LD1 = 48.0 +/- 10.6 %, LD2 = 12.1 +/- 5.2 %, LD3 = 25.9 +/- 7.02 %, LD4 = 4.0 +/- 2.2 % and LD5 = 10.0 +/- 3.5 %. In the activity of some isoenzymes breed differences were recorded in 2-5-year-old ewes (Tabs IV and V). For LD1, significant differences (P < 0.01) were found between the Merino and Ascan Merino x Merino sheep breeds as well as between the Merino x Corriedale sheep and the crossbreds of Merino with a certain share of Lincoln. Differences in LD1 activity between Merino and Merino x Corriedale sheep as well as between the Ascan Merino and Merino crossbreds with a certain share of Lincoln were significant at the level of P < 0.02. For LD2, no significant differences were stated in the pure- and crossbred animals under examination. LD3 values in the Ascan Merino x Merino and Merino x Corriedale sheep significantly differed from those observed in the crossbreds of Merino with a certain genetic share of Lincoln (P < 0.01). Different LD4 and LD5 activities were determined in die Merino and Ascan Merino x Merino sheep (P < 0.02) as well as in the Ascan Merino x Merino and the crossbreds of Merino with a certain share of Lincoln (P < 0.01). In the Merino breed the following LD is isoenzyme activities were recorded: LD1 = 48.5 +/- 9.5, LD2 = 10.1 +/- 3.3 %, LD3 = 28.1 +/- 6.5 %, LD4 = 5.8 +/- 3.5 % and LD5 = 7.5 +/- 6.1 %


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