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Xanthine Oxidoreductase Forms in Tissues of Japanese-Quail

JANKELA J, BARANOVSKA M, ANTALIKOVA J
Veterinarni Medicina 38, 1993, 297-304

The Japanese quail tissues - liver, kidney and pancreas were analysed for the presence of forms of xanthine oxidoreductase utilised cofactors NAD+, molecular oxygen or artificial acceptor - methylene blue, as well as for the validity of correlation between enzymatic activity and diet protein content. Four groups of animals with the experimental diets, the formulae of which are given in Tab. I, and control group with a commercial mash were fed for ten days. For enzyme preparation, the rough purification of cytoplasmic fraction with subsequent dialysis was used. The xanthine oxidoreductase utilised NAD+ (XOR-NAD) was detected in all examined tissues (Fig. 1), whereby the correlation of enzymatic activity with diet protein content was shown only in liver, according our previous findings (Jankela


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