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PAPER SELECTED

Hydrolysis of Cellulose with An Enzymatic Complex from the Digestive Juice of A Garden Snail (Helix-Pomatia)

SMUTNY J, KRIVANOVA M, ZELENAK I, FEJES J
Veterinarni Medicina 37, 1992, 555-560

The method for Observing of the cellulose hydrolysis in vegetable feeds with the enzymatic complex from the digestive juice Of the garden snail Helix pomatia [ECHP] is presented. The reaction rate was observed by measuring the amount of arising reducing sugars according to the method of Somogyi and Nelson. The optimum pH in phosphate buffer was very flat and ranged from 5 to 7. With regard to physiological conditions of hydrolysis in animals and to the aim of our work pH 7 was used. Dependence of the cellulose hydrolysis on the amount of enzyme and substrate form [cellulose] as well as time dependence of hydrolysis were investigated


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