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Applicability of A Skin-Test to the Evaluation of the Actual Level of Cellular-Immunity and Its Level After An Experimental Application of Glucan and Levamisol in Fattening Bulls

PAULIK S, SVRCEK S, BENISEK Z, HUSKA M, DUROVE A
Veterinarni Medicina 37, 1992, 203-212

Applicability of a skin test induced by dinitrofluorobenzene [DNFB] to quantification of the actual level of cellular immunity [CI] in vivo and its level after an experimental immunomodulation intervention were evaluated in two breeds [40 animals in each] of fattening bulls [10-11 months old]. At the selected methodical procedure of intensity determination of the delayed type of hypersensitivity [DTH], its average value reached 4.5 + 1.5 mm in 80 animals, while in 77.5 % of bulls its level ranged from 3.6 to 9.6 mm. in 18.7 % from 2.0 to 3.5 mm and in 3.8 % remained less than 2.0 mm Evident expression of the reaction points to the possibility of application of the used methodical procedure of the skin test using DNFB to quantify the level of CI response in vivo in cattle. Percentual representation of animals according to the intensity of skin reaction [Tab. I] and concentration of total serum immunoglobulins [CS-Ig] and serum IgG [Tab. II] indicates the different cellular and humoral state of animals in investigated breeds. This is also confirmed by the recorded average values of mentioned parameters which were significantly lower [P < 0.01


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